QCQuan


CONSTANd

Just apply CONSTANd (the normalization algorithm used in QCQuan) to your matrix of (aggregated) quantification values. If you have multiple experiments, you need to normalize each matrix separately.
Initial Publication: Maes, E., Hadiwikarta, W. W., Mertens, I., Baggerman, G., Hooyberghs, J., & Valkenborg, D. (2016). CONSTANd: A normalization method for isobaric labeled spectra by constrained optimization. Molecular & Cellular Proteomics, 15(8), 2779-2790.

Upload a csv-file containing only the matrix of quantification values (may contain header and ID column. Delimiters may be ',' or '\t' or ';'):
We recommend using non-transformed intensities as input values. Do not use log2(ratio) values: CONSTANd only works on positive values.
File contains header (first row).
File contains row identifiers (first column).

Found a bug? Send us the details and job ID at qcquan@vito.be.
Terms of Use:
This tool is provided "free to use for all". We made every attempt to ensure the accuracy and reliability of the results provided through this webservice. However, the information is provided "as is" without responsibility or liability of any kind. We don't claim ownership and/or copyright on the input data nor the generated image(s) using our service.